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KMID : 0604520210470020171
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Journal of the Society of Cosmetic Scientists of Korea
2021 Volume.47 No. 2 p.171 ~ p.178
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Skin Barrier Improvement Effect of Exosomal Nanovesicles Derived from Lactic Acid Bacteria
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Wang Hye-Soo
Lee Kwang-Soo
Kang Yong-Won
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Abstract
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In this study, exosomal-like nano-vesicles derived from probiotics were isolated and various physiological activities were evaluated on the skin. This study show that Lactococcus lactis subsp. lactis (LL) are incubated, and then isolated LL derived exosomal nanovesicles (LVs) at the range of 70 ∼ 200 nm by high-pressure homogenizer and ultrafiltration. The vesicle numbers were an average of 1.81 × 1011 particles/mL. This study finds out the bacterial nanovesicles’ beneficial effect on the skin. Fibrillin (FBN1) gene expression increased by 23% in fibroblast cells. Fibronectin (FN1) and filaggrin (FLG) gene expression increased by 65% and 400% in keratinocytes. We could see that cornified envelope (CE) formation ability was increased by 30% compared to the control group. Furthermore, collagen type I alpha 1 (COL1A1) protein expression increased by 83% compared to the UV-irradiated control group. These results suggest that LVs could help skin barrier improvement and used as an ingredient for cosmetics or pharmaceuticals.
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KEYWORD
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probiotics, exosome, nanovesicles, extracellular vesicles, skin barrier
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